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HPLC-ELSD detection of Zhenqi Buxue oral liquid

2024-02-07

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01


Background introduction





Zhenqi Blood-Replenishing Oral Liquid is a traditional Chinese medicine preparation composed of astragalus, sheep placenta, pearl, red ginseng, wolfberry, angelica, coix seed, ginger, jujube and other ingredients. It is suitable for treating dizziness, pale complexion, fatigue, shortness of breath and laziness caused by insufficient qi and blood. Astragaloside IV is one of the main medicinal active ingredients. The content and relative proportion of astragaloside IV directly affect the quality of the sample, and its quality control is particularly important.


With reference to relevant methods, the HPLC-ELSD method was used to establish the detection method of astragaloside IV in Zhenqi Blood-Replenishing Oral Liquid, providing an effective detection solution and providing necessary guarantee for the drug quality control of Zhenqi Blood-Replenishing Oral Liquid.


02


Instruments and equipment





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Instrument:
FCI-2100 liquid chromatography system with ELSD detector
Chromatographic columns:

BISCHOFF PRONTOSIL KROMAPLUS C18, 250×4.6mm, 5μm






03


Test Results









1)Example of a chromatogram of a reference substance test


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10μL Astragaloside Ⅳ Chromatogram


2)Example of chromatogram of the test sample of Zhenqi Buxue Oral Liquid


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20μL test sample chromatogram




3)Detection precautions



The ingredients of Chinese herbal preparations are relatively complex, and there are many small impurities in the test samples. When testing the test samples, after the main component peaks, the chromatographic column must be fully post-balanced, and the next needle should be inserted only after the baseline is completely straight to prevent mutual interference between the two needles.




04


Conclusion





The FCI-2100 series liquid chromatography system equipped with an ELSD detector can be used to separate and detect astragaloside IV and related substances in Zhenqi Buxue Oral Liquid.

05


Configuration List






FCI-2100 liquid chromatography system, equipped with Tongwei UM series ELSD detector

Far-Citech Station Chromatography Workstation

Chromatographic column: C18, 250×4.6mm, 5μm





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