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Highly sensitive proteome-scale searches for cross-linked peptides using CRIMP 2.0

2023-03-20

Cross-linking mass spectrometry (XL-MS) is a valuable technique for generating point-to-point distance measurements in protein space. However, cell-based XL-MS experiments require efficient software to detect cross-linked peptides with sensitivity and controllable error rates. Many algorithms have been developed to reduce the size of the database prior to cross-linking searches using filtering strategies, but there are concerns that using these strategies may reduce sensitivity.

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